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Figure 7 | BMC Neuroscience

Figure 7

From: Requirement of aggregation propensity of Alzheimer amyloid peptides for neuronal cell surface binding

Figure 7

Cell surface aggregation of Aβ. All scale bars are 20 μm in length. (A) Schematic diagram indicating the order of cell surface treatments on NGF differentiated PC12 cells, separated by wash steps indicated by the arrows. (B) Confocal microscopy image of cells treated with 6E10 and secondary Alexa fluor 488 labelled antibody. The absence of staining in this control indicates the primary and secondary antibodies do not non-specifically bind to the cell surface. (C) Images of cells treated with 5 μM unlabelled Aβ42 followed by immunostaining with 6E10 and secondary Alexa fluor 488 labelled antibody. (D) Images of cells treated with 5 μM unlabelled Aβ42 followed by immunostaining, and treatment with 1.5 μM TMR-Aβ42 using 488 nm laser excitation. (E) Images of cells treated with 5 μM unlabelled Aβ42 followed by immunostaining, and treatment with 1.5 μM TMR-Aβ42 using 543 nm laser excitation. (F) Merge confocal microscopy image indicating regions of 6E10 antibody only in green, TMR-labelled Aβ42 in red and co-localized regions in yellow.

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