Tissue distribution of muskelin protein in brain sections of adult mice. (A) Whole brain section of an adult mouse brain. Muskelin displays widespread protein expression with high levels in hippocampus and cerebellum. (B) Within the hippocampus, muskelin-positive cells are found in CA1, CA3 and dentate gyrus regions. (C) Muskelin polypeptides are strongly expressed in cerebellar folia excluding the white matter. (D) Preimmune serum controls represent unspecific signals of the serum and confirm the specificity of signals in A-C. Key: CA1, Hippocampal CA1 region; CA3, Hippocampal CA3 region; Cb, Cerebellum; Cx, Cortex; DG, Dentate gyrus; GCL, Granule cell layer; Hc, Hippocampus; ML, Molecular layer; PCL, Purkinje cell layer; WM, White matter. Scale bars: (A-C) 1 mm.