Figure 4

Expression of CAPS2 splicing variants mRNA in P7 and P21 mouse brains. (A-J) In situ hybridization analysis of the mRNA distribution of CAPS2 isoforms in parasagittal brain sections. (A and B) Exon 11 was used as a probe for CAPS2a, 2b, 2d, and 2e isoforms. (C and D) Exon 22 was used as a probe for the CAPS2b isoform. (E and F) The CAPS2d-specific exon (black rectangle in Figure 2) was used as a probe for the CAPS2d isoform. (G and H) The CAPS2e-specific exon (black rectangle in Figure 2) was used as a probe for the CAPS2e isoform. (I and J) The CAPS2f-specific exon (black rectangle in Figure 2) was used as a probe for the CAPS2f isoform. Scale bar = 2 mm. (K) In situ hybridization analysis of the mRNA distribution of CAPS2 isoforms in the P7 cerebellar cortex. EGL, external granule layer; ML, molecular layer; PCL, Purkinje cell layer; IGL, internal granule layer. Scale bar = 50 μm.