Figure 3

Double immunostaining and immunofluorescence staining. (a) S100B (red)/GFAP (black) double immunolabelling with haematoxylin-counterstaining (blue) revealed distinct populations of cells with astrocytic morphology in the temporal cortex. White arrows indicate S100B single positive cells with astrocytic morphology; black arrows indicate GFAP immunopositive cells with astrocytic morphology. (b) Temporal brain region (overview): The majority of GFAP immunopositive (black) astrocytes were found in the glial limitans and perivascular membrane or in the adjacent white matter. Less GFAP immunopositive cells were observed in the cortex. (c) Temporal cortex and adjacent white matter (higher magnification): S100B positive (red) radial fibres and oligodendrocytes (white arrows) are shown in comparison to GFAP positive (black) astrocytes; haematoxylin-counterstaining (blue). (d) Co-localization (yellow) of S100B (red) and MBP-positive (green) myelinated fibres, extending from the temporal cortex to the adjacent white matter. (e) Co-localization (yellow) of S100B (red) and MOG-positive (green) oligodendrocytes. (f) Co-localization (yellow) of S100B (red) and A2B5-immunopositive (green) O2A glial progenitor cells in the temporal cortex. (g) No co-localization (yellow) was observed for S100B (red) and microglial HLA-DR (green). Bars: a = 100 μm; b = 500 μm; c = 30 μm.