c-Fos and Egr-1 immunoreactivity in WT and CRFR1-/- mice. In terms of c-Fos immunoreactivity (A), significantly lowers levels of cell counts were found in BNSTd in CRFR1-/- relative to WT mice. In terms of Egr-1 immunoreactivity (B), significantly lower levels of cell counts were found in MPOM in CRFR1-/- relative to WT mice. Bars represent means ± SE. White bars indicate WT mice and black bars indicate CRFR1-/- mice. * = p < 0.05; one-way ANOVA. Other abbreviations: AAV = anterior amygdaloid area; AHA = anterior hypothalamic area; PVA = paraventricular nucleus of the thalamus.