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Figure 1 | BMC Neuroscience

Figure 1

From: Dynamic changes in GABAAreceptors on basal forebrain cholinergic neurons following sleep deprivation and recovery

Figure 1

C-Fos expression and GABAAR labeling in BF cholinergic cells across conditions. A. From triple-immunostained sections, a BF cell triple-labeled for c-Fos (with DAB-Ni in gray, A1), ChAT (with Cy2 in green) and β2–3 GABAAR (with Cy3 in red, A2) from an SD brain. Note prominent GABAAR labeling over the plasma membrane of the cell. B. From dual-immunostained sections, a BF cell double-labeled for ChAT (with Cy2, B1) and β2–3 GABAAR (with Cy3, B2) from an SD brain and showing the prominent labeling along the membrane of the soma and proximal dendrites along with the boxes (in yellow) that were used for luminance measurements of the GABAAR labeling over the plasma membrane (on two sides) and the nucleus. The luminance of the nucleus, which was considered to represent and thus control for nonspecific, background fluorescence, was subtracted from the mean luminance of the membrane of each cell as a measure of intensity membrane GABAAR (Fig. 3). C-E. BF cells dual-immunostained for ChAT (Cy2, C1, D1, E1) and β2–3 GABAAR (Cy3, C2, D2, E2) from representative brains (selected according to mean values of membrane intensity per condition) of SC, SD and SR groups (Fig. 3). Scale bar, 20 μm. Abbreviations: SC, sleep control; SD, sleep deprived; SR, sleep recovery.

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