Figure 3

Effect of glutamate on RGC morphology and survival. Cells were treated with glutamate or vehicle for 3 days. (A and B) Morphology of RGCs with glutamate (100 μM) treatment. (A) The cells were labeled with anti-Thy-1 antibody (red) and DAPI nuclear stain (blue). (B) The corresponding phase-contrast image. (C) Effect of glutamate (100 μM) on RGC survival (n = 76). *** represents P < 0.001 vs. the control group by Student's t-test. (D) Concentration-dependent toxicity of glutamate on cultured RGCs (n = 6). * represents P < 0.05 vs. the control group by One-way ANOVA then Dunnett's test. (E) Effect of MK801 on glutamate-induced RGC toxicity. Cells were treated with the indicated concentrations of MK801 in the presence of 100 μM of glutamate (n = 6). * represents P < 0.05 vs. the glutamate alone group by One-way ANOVA then Dunnett's test. Cell survival in (C), (D), and (E) was assessed by manually counting the Thy-1-positive cells. Data are presented as mean ± SEM.