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Table 4 Quantitative real-time PCR (qPCR) does not support Sema3A-induced changes in SCG observed by microarray.

From: Analysis of gene expression during neurite outgrowth and regeneration

Gene

Gene Title

Max. difference by microarray§

Max. difference by qPCR

p-value

Col18

Procollagen, type XVIII, alpha 1

2.7

2.5

0.66

Cryab*

Crystallin, alpha B

2.5

2.2

0.21

Fdps

Farnesyl diphosphate synthetase

2.0

2.3

0.22

L05*

RIKEN cDNA 1110035L05 gene

4.3

1.5

0.82

Lmo4*

LIM domain only 4

2.1

1.6

0.26

Ptprm

Receptor protein tyrosine phosphatase M

2.7

2.8

0.27

Rce1

Ras and a-factor-converting enzyme 1 homolog

1.4

1.3

0.77

Timp3*

Tissue inhibitor of metalloproteinase 3

1.7

2.6

0.18

Unc119

unc-119 homolog

1.8

1.3

0.95

Uty*

Ubiquitously transcribed gene, Y chromosome

3.4

2.6

0.33

  1. Italicized p-values indicates lack of statistical significance of qPCR data for a quadratic model of change over time.
  2. * Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
  3. § Mock-Sema3A differences observed by microarray were statistically significant (quadratic model; p ≤ 0.05).
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BMC Neuroscience

ISSN: 1471-2202

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