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Table 4 Quantitative real-time PCR (qPCR) does not support Sema3A-induced changes in SCG observed by microarray.

From: Analysis of gene expression during neurite outgrowth and regeneration

Gene Gene Title Max. difference by microarray§ Max. difference by qPCR p-value
Col18 Procollagen, type XVIII, alpha 1 2.7 2.5 0.66
Cryab* Crystallin, alpha B 2.5 2.2 0.21
Fdps Farnesyl diphosphate synthetase 2.0 2.3 0.22
L05* RIKEN cDNA 1110035L05 gene 4.3 1.5 0.82
Lmo4* LIM domain only 4 2.1 1.6 0.26
Ptprm Receptor protein tyrosine phosphatase M 2.7 2.8 0.27
Rce1 Ras and a-factor-converting enzyme 1 homolog 1.4 1.3 0.77
Timp3* Tissue inhibitor of metalloproteinase 3 1.7 2.6 0.18
Unc119 unc-119 homolog 1.8 1.3 0.95
Uty* Ubiquitously transcribed gene, Y chromosome 3.4 2.6 0.33
  1. Italicized p-values indicates lack of statistical significance of qPCR data for a quadratic model of change over time.
  2. * Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
  3. § Mock-Sema3A differences observed by microarray were statistically significant (quadratic model; p ≤ 0.05).