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Table 2 Validation using quantitative real-time PCR (qPCR) of genes found to change significantly in SCG by microarray.

From: Analysis of gene expression during neurite outgrowth and regeneration

Gene Gene Title Max. fold change by microarray§ Max. fold change by qPCR p-value
Col18 Procollagen, type XVIII, alpha 1 2.0 2.3 0.59
Fdps Farnesyl diphosphate synthetase 1.8 2.3 0.05
Itga4 Integrin alpha 4 2.8 5.3 0.05**
L05* RIKEN cDNA 1110035L05 gene 6.8 5.4 0.02
Ptprm Receptor protein tyrosine phosphatase M 2.7 5.6 0.008
Timp3* Tissue inhibitor of metalloproteinase 3 2.1 2.1 0.25
  1. p-value indicates significance of qPCR data for a quadratic model of change over time. Italicized p-values for two genes indicate lack of statistical significance.
  2. * Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
  3. ** Significance for this gene is for a linear model of change over time; this gene does not fit a quadratic model.
  4. § Changes observed by microarray were statistically significant (quadratic model; p ≤ 0.05).