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Table 2 Validation using quantitative real-time PCR (qPCR) of genes found to change significantly in SCG by microarray.

From: Analysis of gene expression during neurite outgrowth and regeneration

Gene

Gene Title

Max. fold change by microarray§

Max. fold change by qPCR

p-value

Col18

Procollagen, type XVIII, alpha 1

2.0

2.3

0.59

Fdps

Farnesyl diphosphate synthetase

1.8

2.3

0.05

Itga4

Integrin alpha 4

2.8

5.3

0.05**

L05*

RIKEN cDNA 1110035L05 gene

6.8

5.4

0.02

Ptprm

Receptor protein tyrosine phosphatase M

2.7

5.6

0.008

Timp3*

Tissue inhibitor of metalloproteinase 3

2.1

2.1

0.25

  1. p-value indicates significance of qPCR data for a quadratic model of change over time. Italicized p-values for two genes indicate lack of statistical significance.
  2. * Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
  3. ** Significance for this gene is for a linear model of change over time; this gene does not fit a quadratic model.
  4. § Changes observed by microarray were statistically significant (quadratic model; p ≤ 0.05).
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BMC Neuroscience

ISSN: 1471-2202

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