Comparison of gene expression data by microarray analysis and qPCR. Time course of gene expression change in DRG (A) and in SCG (B) for genes with statistically significant change by both microarray and qPCR (p ≤ 0.05; see Tables 1–2 for gene names and details). Solid lines plot the quadratic fit of Affymetrix intensity values over time, while the dashed line represents the quadratic fit of the qPCR log ratios. All data is expressed in a log2 scale. qPCR data are normalized to a common reference gene (Wdr4; see Methods for details) and plotted relative to the 2 hour starting point.