Scheme of a model signaling network. Scheme of the signaling network used to demonstrate the validity of the parameter estimate method. The network consists of a series of proteins (the nodes) linked by different types of unary, binary or multiple molecular interactions (shown as the edges of the network). The role of the mitochondrion (in purple) is taken into account. Binding protein-protein interactions are shown by green edges between the nodes, activation and deactivation interactions are in blue and red, respectively, chemical transformations are shown by purple dotted lines, while the release of proteins from the mitochondria in shown in solid purple lines. The signaling process can be activated by the binding of ligands (in grey) to receptors. Every compound is identified by a name and a numerical code.