Effects of lipopolysaccharide-induced inflammation on expression of growth-associated genes by corticospinal neurons

Table 3 Quantification of CST axons

	Lesion site axon total	Branching points	Medulla axon total	Total sprouting ratio	Lesion site sprouting ratio
LPS 1	133	52	466	0.11	0.39
LPS 2	123	100	788	0.13	0.81
LPS 3	146	54	685	0.08	0.36
LPS 4	301	128	519	0.25	0.42
LPS group mean ± SEM			614.5 ± 74.5	0.14 ± 0.04	0.5 ± 0.1
Con 1	150	162	998	0.16	1.08
Con 2	273	162	602	0.27	0.59
Con 3	200	78	323	0.24	0.39
Con 4	138	98	439	0.22	0.71
Control group mean ± SEM			590.5 ± 147.5	0.22 ± 0.02	0.69 ± 0.15
Student's t-test p value for LPS-treated vs. control animals			0.89	0.12	0.32

Notes: BDA-labelled axons (and branch points) in 4 LPS-treated animals and 4 control animals (LPS not applied) were counted at two sites: 1) in 9 horizontal sections through the cervical spinal cord 0.4 mm rostral to the lesion site, in a 60 μm wide counting frame (labelled axons and branch points); 2) in transverse sections through the medulla (number of labelled axons in pyramid). Total sprouting ratio is the ratio between the number of branch points and the total number of labelled CST axons at the level of the medulla. Lesion site sprouting ratio is the ratio between branch points and labelled axons at the same level (0.4 mm rostral to the lesion site); SEM = standard error of the mean

ISSN: 1471-2202