Figure 2

Editing in newly collected flies, in different tissues, and at different development stages. The base modified by RNA editing is boxed. (A). The genomic sequence at the boundaries of intron 4 from four different wild-type strains newly collected in central Germany. The black letters give sequences from exons 4 and 5, grey letters belong to intron 4. The corresponding amino acids are shown underneath the codons. The protein kinase target motif at the genomic sequence is in all cases RRFS (bold letters). (B). cDNA sequences from the lines in A). In all strains editing of a genomic adenosine to a guanosine in the cDNA leads to the modified motif RGFS (bold letters). (C). cDNAs from different tissues and different development stages. In both head and body homogenates the RNA modification was detected. RNA editing is also found during all development stages. In all cases RNA editing appears to modify all pre-mRNA except in the fraction of eggs plus first instar larvae where an adenosine peak overlaps in the sequence record with the G (asterisk).