Figure 4

17β-estradiol prevents Amyloid beta_1–42-induced decline in mitochondrial Bcl-2 expression and Bax translocation. Primary hippocampal neurons pretreated with E₂ (10 ng/mL; 48 hr) or vehicle control were exposed to Aβ_1–42 for 24 hr and mitochondrial fractions were assessed for (A) Bcl-2 and (B) Bax expression by Western blot analysis. (* = p < 0.05 as compared to control; ** = p < 0.01 as compared to control; + = p < 0.05 as compared to Aβ_1–42 alone; n = 4).