Figure 3
17β-estradiol prevents Amyloid beta1–42-induced rise in resting calcium concentration. Primary hippocampal neurons pretreated with E2 (10 ng/mL; 48 hr) or vehicle control were exposed to Aβ1–42 for 24 hr and assessed for cytosolic calcium concentration by Fura2 imaging. A. Representative images of Fura2 fluorescence in psuedocolor representing 340/380 ratio. B. Quantitative changes in Fura2 340/380 ratio presented as means +/- S.E.M. (** = p < 0.01 as compared to control; ++ = p < 0.01 as compared to Aβ1–42 alone; n = 4).