Figure 5

western blotting analysis and quantification of the percentage of AMPA and NMDA receptor subunits expression in spinal cord of wobbler mice. Representative immunoblot from cervical spinal cord samples from 12-week-old healthy mice and age-matched wobbler mice. Left column: whole spinal cord homogenates. Right column: spinal cord TIF. Quantification of the mean values of immunodensity for CaMK, AMPA and NMDA receptor subunits, obtained from the cervical spinal wobbler mice in whole spinal cord homogenates (Homo) and in TIF. Data are representative of two independent experiments on two different homogenate preparations of four pooled animals for each group and replicated two times in each homogenate preparation. Values reported represent the percentage of the mean values of immunodensity obtained in 12-week-old wobbler mice compared to the levels measured in healthy littermates and normalized to 100. Both preparations samples were analyzed by Western blot analysis with CaMKII, GluR1, GluR2, GluR3, GluR4, NR1, and NR2A antibodies. The same amount of protein was loaded per lane.