ionotropic glutamate receptors in the lamina IX in the cervical spinal spinal cord of four-week-old wobbler mice. UPPER PANELS. Representative photomicrographs showing the patterns of GFAP immunostaining in the cervical spinal cord region of 12-week-old mice (A) and age-matched symptomatic wobbler mice (B). CENTRAL PANELS (C-V). Triple staining experiments using Nissl (C, G, K, O, S, purple colour) GFAP (D, H, L, P, T, red colour) and GluR 1–4 or NR2A (E, I, M, Q, U, green colour) in the anterior horn of four-week-old wobbler mice. The merge between the three different colours is shown in panels F, J, N, R, V. LOWER PANELS (W-Z). Triple staining showing Nissl (W), GFAP (X), GluR4 (Y) and the merge between the three different colurs (Z), in the white matter in the anterior region of the cervical spinal cord, four-week-old wobbler mouse. Scale bar, A-B, 100 μm. C-V, 30 μm. W-Z 40 μm.