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Figure 11 | BMC Neuroscience

Figure 11

From: Progressive ganglion cell loss and optic nerve degeneration in DBA/2J mice is variable and asymmetric

Figure 11

Summary of the scoring criteria for DiI-labeled nerves and X-gal stained retinas. (A) Exemplar of scored optic nerves. Only the left nerve is shown for 5 individual mice. The scores range from 1 for label from the globe to the chiasm, to 5 for no signs of label. Younger mice typically exhibited nerves that were scored 1–2, while older mice typically had nerves showing some level of degeneration (3–5). Bar = 0.5 mm. (B) A photomicrograph of a retina (OD) stained for βGEO activity taken from a 10.5 month old mouse. This particular example appears to have 2 wedges of cell loss, one superior and one temporal, at different stages of degeneration. (C) A cartoon of a flatmounted retina where each quadrant is separated into 4 regions and given a score based on the intensity of stain present. The quadrants represented are (clockwise): SN, superonasal; IN, inferonasal; IT, inferotemporal; ST, superotemporal. The scores in each region of the quadrants represent the scores given by 1 masked observer for the retina in (B). The average of all the scores in each quandrant then becomes the total score for that quadrant, and the average of these 4 scores becomes the final total score for that retina.

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