PMBSF Procrustes scatterplot. Procrustes superimposition of 26 barrel landmarks from each of 13 adult C57BL/6J mice and 12 adult DBA/2J mice. C57BL/6J is shown in blue circles. DBA/2J is shown in red squares. Blue triangles show C57BL/6J strain means. Red stars show DBA/2J strain means. Barrel identity is indicated as labeled. The figure shows the actual spread in the superimposed data, but also indicates the shape differences. One can readily discern strain differences in the position of any barrel by noting the relative separation by color for any given landmark point cloud. For example, in barrel A1, the red squares (DBA/2J) are lateral of the blue circles (C57BL/6J) and show no overlap. A similar lateral difference is seen in the point clouds for barrels A2 and A3, although the blue circles become progressively shifted anteriorly. See Figure  and text for a more complete description of noted shape changes. Figure  was composed in TwoGroup, a shape software title in the IMP series described in .