Figure 8

Theta pulse-induced LTP in hippocampal slicesrecorded by 5 × 13 3D MEAs. A) LTP was induced by 150 pulses given at 5 Hz in hippocampal slices from 129S5 (n = 15) and C57 (n = 13) mice. During TPS, amplitude of stimulation was 1.5× of the baseline strength. In the first five minutes following TPS, a transient depression was seen in both control and test pathways (see Additional file 2). Therefore, first five records after TPS were omitted from the plot for clarity. In some cases, TPS did not produce persistent facilitation of fEPSP amplitude that returned to baseline levels after 60 min. However, we pooled data from all slices that exhibited pronounced complex spikes during TPS (see Additional file 2). B) Inhibition of NMDA receptors by 50 μM D,L-AP5 led to a significant attenuation of TBS-induced LTP in 5 slices from 129S5 mice. Average amplitude of normalised fEPSP 60 min after TBS was significantly less in D,L-AP5-treated slices compared to untreated 129S5 cohort (110 ± 9% vs. 138 ± 20 %; t₍₁₉₎ = -3.25; P < 0.01).