Figure 7

Theta burst-induced LTP in hippocampal slicesrecorded by 5 × 13 3D MEAs. A) Summary LTP data obtained in slices from 129S5 (n = 14) and C57 (n = 7) mice. Exploiting two-pathway paradigm, we corroborated data from one-pathway experiments (Figs. 3B,4A) on robust LTP induced by TBS. No up-regulation of the stimulus strength during TBS was done when using 5 × 13 3D MEAs. B) Inhibition of NMDA receptors by 50 μM D,L-AP5 led to a significant attenuation of TBS-induced LTP in 5 slices from 129S5 mice. Average amplitude of normalised fEPSP 60 min after TBS was significantly less in D,L-AP5-treated slices compared to untreated 129S5 group (109 ± 5% vs. 178 ± 35 %; t₍₁₇₎ = -4.24; P < 0.001).