Examination of the frequency of HDGF and HRP-3 expressing cells in the cerebellum. Antibodies against HDGF (A, C) or HRP-3 (D, F) were used to detect both proteins in the cerebellum of an adult mouse. To label the nuclei of all cells in the sections counterstaining with propidium iodide was performed (B,C and E,F). Double stained sections were used to calculate the percentage of cells positive for HDGF or HRP-3, respectively. Whereas HDGF is expressed in all cells, HRP-3 expression is much more restricted (for details see text). Cells labeled with arrows in D represent HRP-3 positive Purkinje cells. Asterisks in E mark most likely radial glia cells. MCL: molecular cell layer; PCL: purkinje cell layer; IGL: internal granular cell layer; Bars are 25 μm.