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Figure 5 | BMC Neuroscience

Figure 5

From: Reduced neuronal cell death after experimental brain injury in mice lacking a functional alternative pathway of complement activation

Figure 5

Upregulation of Bcl-2 and downregulation of Fas receptor in brains of fB -/- mice after traumatic brain injury (TBI), as determined by Western blot analysis. Homogenized brain tissue specimens from the injured hemispheres of sham-operated and head-injured fB+/+ and fB-/- mice were run out on SDS-PAGE, transferred to nitrocellulose membranes, and analyzed with specific monoclonal antibodies to Bcl-2, Fas, and β-actin as internal control and detection by chemiluminescence assay (ECL® system, Amersham). (A) The visualized 26 kDa band corresponding to mouse Bcl-2 is enhanced in the knockout mice at 24 hours, compared to head-injured wild-type littermates. Furthermore, a downregulation in Fas receptor staining intensity is obvious in brain-injured knockout mice at all time-points assessed, compared to fB+/+ mice. The exemplary blot is representative of three independent experiments. (B) The individual band intensities of the blot shown in panel A were quantified by TINA 2.09 software (Raytest, Straubenhardt, Germany) and the data are shown as relative levels to the according β-actin band intensity.

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