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Figure 1 | BMC Neuroscience

Figure 1

From: Reduced neuronal cell death after experimental brain injury in mice lacking a functional alternative pathway of complement activation

Figure 1

Screening of serum samples from fB -/- and fB +/+ mice for factor B protein, as shown for an exemplary Western blot. Samples were run out on SDS-PAGE, transferred to nitrocellulose membranes, and analyzed with a specific monoclonal anti-mouse factor B antibody and detection by chemiluminescence (ECL® system, Amersham). A specific 98 kDa band, corresponding to murine fB, is detectable in the fB+/+, but not in the fB-/- mice. Each lane is representative of an individual mouse. The bottom panel shows equal protein loading in the different bands by ponceau red staining.

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BMC Neuroscience

ISSN: 1471-2202

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