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Figure 7 | BMC Neuroscience

Figure 7

From: Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells

Figure 7

The tyrosine kinase inhibitor herbimycin A blocked IGF-1 signalling, including Akt and CREB activation, in PC12 cells. PC12 cells pretreated with or without herbimycin A were stimulated with 10 nM IGF-1 and the tyrosine phosphorylation of IGF-1 R (A) and IRS-1 (B), their association with PI3K, as well as the phosphorylation of Akt and CREB (C) were determined. IGF-1 stimulated the tyrosine phosphorylation of these markers. Pretreatment of PC12 cells with herbimycin A blocked these events. Blots represent prototypical example of experiments replicated at least 3 times.

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BMC Neuroscience

ISSN: 1471-2202

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