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Figure 4 | BMC Neuroscience

Figure 4

From: Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells

Figure 4

The MEK kinase inhibitor PD98059 attenuated IGF-1-induced activation of MAPK kinase and CREB in PC12 cells. PC12 cells pretreated with various concentrations of the MAPK pathway inhibitor PD98059 were incubated with 10 nM IGF-1 for 10 min. Cells were collected and the activation of MAPK and CREB were determined as described in Methods. PD98059 concentration-dependently blocked the activation of MAPK stimulated by IGF-1. This inhibitor also attenuated the phosphorylation of CREB in a similar manner. Blots represent prototypical example of experiments replicated at least 3 times.

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