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Figure 3 | BMC Neuroscience

Figure 3

From: Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells

Figure 3

PI3 kinase inhibitors blocked the activation of Akt by IGF-1 while has no effect on the phosphorylation of CREB. PC12 cells pretreated with different concentrations of LY294002 or 100 nM wortmannin were stimulated with 10 nM IGF-1 and the phosphorylation of Akt and CREB was determined. (A) LY294002 blocked IGF-1-induced phosphorylation of Akt in a concentration-dependent manner while slightly enhancing the activation of CREB in PC12 cells. (B) 100 nM wortmannin significantly blocked the activation of Akt while having no effect on CREB phosphorylation. Blots represent prototypical example of experiments replicated at least 3 times.

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