Figure 2

Neurosphere stem cell lineages express PTPσ. a) A neurosphere derived from the SVZ of a PTPσ(+/-) mouse brain and reacted for the histochemical localization of β-galactosidase. Nomarski phase contrast inverted microscopy. Scale bar = 50 μm. b) Histochemical localization of the PTPσ reporter gene β-galactosidase in neurospheres originating from a PTPσ(-/-) mouse. Phase contrast inverted microscopy. Scale bar = 25 μm. c) Co-expression of the progenitor cell marker NG2 (green) and the neuronal lineage marker tubulin (red) in cells differentiated from a PTPσ(-/-) mouse. Epi-fluorescence microscopy. Scale bar = 50 μm. d) Differentiated neurons derived from a PTPσ(-/-) neurosphere. The merged image shows the co-expression (yellow) of β-galactosidase and β-III tubulin in the cytoplasm. Epi-fluorescence microscopy. Scale bar = 50 μm. e) Astrocytes contain GFAP (rhodamine-red) and β-galactosidase (fluorescein-green) when differentiated from a PTPσ(-/-) neurosphere. Epi-fluorescence microscopy. Scale bar = 50 μm. f) Mature oligodendrocytes derived from a PTPσ(-/-) mouse stained with the 04 antibody. Epi-fluorescence microscopy. Scale bar = 50 μm.