Figure 6

Effect of calpain and caspase inhibitors on glutamate-induced DNA fragmentation (DNA ladder) in HT22 cells. HT22 cells were treated with 8 mM glutamate in the presence or absence of calpain inhibitors 25 μM ALLN and 100 μM PD150606, and caspase inhibitors 100 μM z-DEVD and 100 μM z-VAD. A: HT22 cells were treated with glutamate in the presence of calpain inhibitors for 8 hrs. Lane 1 = untreated control cells; Lane 2 = glutamate alone; Lane 3 = glutamate + PD150606; Lane 4 = PD150606 alone; Lane 5 = glutamate + ALLN; Lane 6 = ALLN alone. B: HT22 cells were treated with glutamate in the presence of caspase inhibitors for 16 hrs. Lane 1 = untreated control cells; Lane 2 = z-DEVD alone; Lane 3 = z-VAD alone; Lane 4 = glutamate alone; Lane 5 = glutamate + z-DEVD; Lane 6 = glutamate + z-VAD; M: 100 bp DNA marker. Gel electrophoresis was carried out as described under "Methods" and the gels were stained with ethidium bromide.