Figure 3

Effect of calpain and caspase inhibitors on glutamate toxicity in HT22 cells as assessed by phase contrast microscopy (20×). HT22 cells were treated with 8 mM glutamate in the presence or absence of calpain inhibitors, 25 μM ALLN and 100 μM PD150606, and caspase inhibitors, 100 μM z-DEVD and 100 μM z-VAD, for 8 hrs. Normal cells are depicted by arrows and degenerated cells by arrowheads. A = untreated control cells; B = glutamate alone; C = glutamate + ALLN; D = glutamate + PD150606; E = glutamate + z-DEVD; F = glutamate + z-VAD.