Effects of TMP on Bax expression in spinal cord at the 48th hour reperfusion. Immunohistochemical photomicrographs (magnification × 400) of anterior horn tissue stained for Bax protein in sham group (A), control group (B) and TMP group (C). Immunostaining was performed using a specific anti- Bax antibody and developed with stable DAB. The positive staining of Bax is presented by a brown color of cytoplasm. Figures are representative of 3 separate experiments with similar results.