Effects of TMP on cell apoptosis in the spinal cord at the 48th hour reperfusion. Representative images of TUNEL staining (magnification × 200) in sham group (A), Control group (B) and TMP groups (C). Quantitative analysis of apoptosis rate (D). Cell apoptosis was determined using TUNEL staining as described under "Methods". Cell apoptosis rate is expressed as the mean ± S.D. from three experiments. *P < 0.01, vs sham group; **P < 0.01, vs control group.