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Figure 3 | BMC Neuroscience

Figure 3

From: Chemosensory properties of murine nasal and cutaneous trigeminal neurons identified by viral tracing

Figure 3

Identification of infected cells in the TG. (A) After inoculation of PrV-B80 to the right nostril X-gal staining identified β-gal expressing cells from a dorsal view at 40 hpi in superficial layers of the dorsomedial portion of the ipsilateral TG. (B) At 60 hpi cells appeared also in other regions (arrow head). Dashed lines indicate assumed regions with cells of ethmoidal origin. The schematic overviews compile infected cells of seven TGs each (infected cells from different animals are indicated by dots of different colors). (C) 3D multiphoton microscopy identified infected areas 48 hours after inoculating PrV-Cam exclusively in the dorsomedial portion of the ganglion. Viral invasion was mediated most likely by transport via the peripheral collaterals of neurons, since the morphology of labeled cells (A, inlay) and the speed of transportation from the nasal cavity to the trigeminal ganglion tend to exclude the involvement of glial cells. Bars in A: 200 μm, inlay 40 μm, C: 50 μm

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