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Figure 2 | BMC Neuroscience

Figure 2

From: Activation of MAPK ERK in peripheral nerve after injury

Figure 2

Immunoreactivity of phospho-ERK (ERK-P) in the intact and proximal stumps of crushed sciatic nerves. The nerves were longitudinally sectioned (C, D, F, G and H) or transversely sectioned (A, B and E). The sections exposed to secondary antibodies conjugated with FITC or rhodamine without primary antibodies are shown in A and B, respectively. C-E were from intact nerves. F-H were representatives of the proximal stumps from all time points studied. Single staining for ERK-P only (C, E and F). Double staining for ERK-P (green) and pan-neurofilament (NF, red) (D & G). Double labeling for ERK-P (green) and S-100 (red) (H). Arrowheads indicate the locations of axons; filled arrows indicate the ERK-P immunoreactivity in Schwann cells. In the intact nerve, ERK-P was present in the axons as indicated by co-localization with pan-neurofilament (C & D), and Schwann cells as recognized by the characteristic semilunar shape (E). In the proximal stump, ERK-P was exclusively co-localized with pan-neurofilament in the axons (F & G), but not Schwann cells (open arrows in H). Scale bars represent 40 μm.

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