Figure 1

Phosphorylation of ERK in sciatic nerves at various time points after nerve crush. Sample immunoblots probed for ERK-P and ERK-T are shown above. For ERK-P, two isoforms were observed (ERK1 at 44 kDa and ERK2 at 42 kDa), whereas they were not distinguishable in ERK-T. The bar chart below demonstrates the ratio of ERK-P to ERK-T in three nerve segments for each time point. The data are means ± SEM. AU = arbitrary unit, D1 = day 1, W1 = week 1, W2 = week 2, M1 = month 1, I = intact nerve, P = proximal segment to the crush lesion, D = distal segment to the crush lesion. * p < 0.05 vs. W1 intact, # p < 0.01 vs. W1 intact, ** p < 0.05 vs. M1 proximal and p < 0.01 vs. M1 intact, * and # by Kruskal-Wallis test, ** by ANOVA, n = 6–7 for each time-point.