Table 1 Primary and secondary antisera.
a. Primary Antisera | ||||
|---|---|---|---|---|
Antigen | Host | Â | Source | Working Dilution |
GFRα1 | Goat |  | R&D Systems (Minneapolis, MN, USA) | 1:200 |
GFRα2 | Goat |  | R&D Systems | 1:200 |
Synapsin | Rabbit | Â | Biogenesis (Poole, UK) | 1:1000 |
Synaptophysin | Mouse | Â | Dako (Botany, NSW, Australia) | 1:200 |
Tyrosine hydroxylase | Mouse | Â | DiaSorin (Stillwater, MN, USA) | 1:2000 |
Vasoactive intestinal peptide | Rabbit | Â | Dia Sorin | 1:2000 |
b. Secondary Antisera | ||||
Antigen | Host | Conjugate | Source | Working Dilution |
Goat IgG | Donkey | Cy3 | Jackson (West Grove, PA, USA) | 1:1000 |
Mouse IgG | Horse | Biotin | Vectorlabs (Burlingame, CA, USA) | 1:200 |
Rabbit IgG | Donkey | FITC | Jackson | 1:50 |