Effects of axotomy on intensity of GFRα1immunofluorescence in rat pelvic ganglia. a. Immunofluorescence intensity measurements showed that GFRα1 immunoreactivity was not affected in injured FluoroGold (FG) but decreased slightly in noradrenergic neurons (TH; P = 0.046). Data were obtained from 5 rats that underwent unilateral penile nerve transection, with matched data from left (injured) and right (control) ganglia from the sameanimal. Panels b and c show matched pairs of images from sections double-stained for GFRα1 (left) and VIP (right). b, contralateral (control) ganglion. c, ipsilateral (injured) ganglion. Calibration bar represents 20 μm.