Downregulation of ZAS3 in SpNL. (A) Upper panels, RT-PCR transcript analyses of ZAS1, ZAS2, ZAS3, NGF and actin of DRG seven days after SpNL nerves (top panel). The experiments were carried out as described in the text (n = 4 per group) and a representative result is shown. (Bottom panel) Bar charts showing relative levels of each PCR products adjusted with actin in the same cDNA sample. Significance (*) is p < 0.05. (B) Immunoblot analyses of total protein lysates in ipsilateral (I) DRG and contralateral (Con) DRG prepare from rats seven days after surgery using AbC (a), AbN (b), and p65 antibodies (c). Asterisk indicates the 200 kDa species observed only in contralateral DRG but not in ipsilateral DRG. (C) Immunoblot analysis of cytoplasmic and nuclear extracts with p65 and histone H1 antibodies.