Expression of ZAS3 in motoneurons of lumbar spinal cord. Triple fluorescent ZAS3, GFAP, and DAPI staining of lumber spinal cord sections. Many cell nuclei were devoid of ZAS3 (circled), suggesting that the antisera, AbC and AbN, were specific. While AbC stained the nuclei of motoneurons, AbN stained both the nuclei and cytoplasm of motoneuorns and astrocytes. When the images of AbN and GFAP are superimposed, cells that expressed both proteins are yellow (highlighted with a white arrow in A, C, and E). When the images of AbC and DAPI are superimposed, nuclei expressing ZAS3 appear purple (highlighted with a yellow arrow), whereas nuclei that did not express ZAS3 are blue (circled).