Primary structure of ZAS3 and expression of ZAS3 in the trigeminal ganglion and dorsal root ganglia. (A) Schema showing the domain structure of the ZAS3 protein and fusion proteins used to raise polyclonal antisera, AbN and AbC. Black boxes, zinc finger(s); grey box, middle conserved region; ZAS, zinc finger, acidic- and serine/threonine-rich regions; S, S protein; and mbp, maltose binding protein. (B) and (C) Immunohistochemical staining of sections of naïve rat trigeminal ganglion using antisera AbN and AbC, respectively. Inserts are higher magnifications showing uniform cellular staining of AbN and prominent nuclear staining with AbC. Bars represent 200 μM. (D) and (E) Longitudinal sections of DRG immunostained with AbN and AbC, respectively. Arrows highlight differential nuclear staining of AbN and AbC. Bars represent 100 μM.