Synaptic depression of auditory fibers does not interfere with synaptic depression of trigeminal afferents. A: 100 bursts were applied at 1 Hz first to the trigeminal and immediately afterwards to the auditory fibers in rats (n = 7). Both burst sequences induced considerable synaptic depression in the respective pathway (general linear model: p < 0.0001), showing that the induction of synaptic depression in one pathway did not interfere with subsequent synaptic depression in the other pathway. B: This protocol was repeated in C57/BL6 mouse slices (n = 5). Again, both sequences induced synaptic depression in the respective pathway. C: After a recovery period, both sequences of 100 bursts were again applied, but first to the auditory and then to the trigeminal pathway. The first trigeminal cEPSCs of sequences 1 and 3 as well as the first auditory cEPSCs from sequences 2 and 4 were not statistically different.