| Lenght of amplified | Primer sequences | Cycles |
---|---|---|---|
Nestin | 220 bp | 5'GCCCTGACCACTCCAGTTTA3' 5'GGAGTCCTGGATTTCCTTCC3' | 33 cycles: 30 sec, 94°C 30 sec, 55°C 30 sec, 72°C |
Vimentin | 200 bp | 5'GGGACCTCTACGAGGAGGAG3' 5'CGCATTGTCAACATCCTGTC3' | 30 cycles: 30 sec, 94°C 30 sec, 55°C 30 sec, 72°C |
NF-M | 333 bp | 5'TGGGAAATGGCTCGTCATTT3' 5'CTTCATGGAAGCGGCCACTT3' | 35 cycles: 30 sec, 94°C 60 sec, 55°C 90 sec, 72°C |
NSE | 329 bp | 5'TGAACACAGACGCTATGCGCTCAG3' 5'CACCTTTATGTGAGTGGACACAGA3' | 30 cycles: 60 sec, 94°C 60 sec, 60°C 120 sec, 72°C |
Hes1 | 349 bp | 5'ACACGACACCGGATAAACCAA3' 5'CGAGTGCGCACCTCGGTA3' | 25 cycles 30 sec, 94 °C 30 sec, 60 °C 30 sec, 72 °C |
GAPDH | 347 bp | 5'GCCAAAAGGGTCATCATCTCTG3' 5'CATGCCAGTGAGCTTCCCGT3' | 25 cycles: 30 sec, 96°C 60 sec, 58°C 30 sec, 74°C |