Table 1 Plating parameters.
From: An extremely rich repertoire of bursting patterns during the development of cortical cultures
'Dense' | 'Small' | 'Sparse' | 'Small & sparse' | 'Ultra sparse' | |
|---|---|---|---|---|---|
Plating volume (μL) | 20 | 5 | 20 | 5 | 20 |
Density of suspension (cells/μL) | 2500 | 2500 | 625 | 625 | 156 |
Number of cells plated (nominal) | 50,000 | 12,500 | 12,500 | 3,125 | 3,125 |
Culture diameter (mm)a | 4.9 ± 0.4 | 3.1 ± 0.3 | 4.9 ± 0.4 | 3.1 ± 0.3 | 4.9 ± 0.4 |
Drop thickness (mm)a,b | 1.69 ± 0.24 | 1.06 ± 0.23 | 1.69 ± 0.24 | 1.06 ± 0.23 | 1.69 ± 0.24 |
Density at 1 div (×103 cells/mm2)c | 2.5 ± 1.5 | 1.6 ± 0.6 | 0.60 ± 0.24 | 0.30 ± 0.16 | 0.11 ± 0.06 |
Number of cultures followed | 30 | 12 | 10 | 3 | 3 |
Number of batches | 8 | 3 | 3 | 1 | 1 |