Skip to main content

Advertisement

Table 1 Plating parameters.

From: An extremely rich repertoire of bursting patterns during the development of cortical cultures

  'Dense' 'Small' 'Sparse' 'Small & sparse' 'Ultra sparse'
Plating volume (μL) 20 5 20 5 20
Density of suspension (cells/μL) 2500 2500 625 625 156
Number of cells plated (nominal) 50,000 12,500 12,500 3,125 3,125
Culture diameter (mm)a 4.9 ± 0.4 3.1 ± 0.3 4.9 ± 0.4 3.1 ± 0.3 4.9 ± 0.4
Drop thickness (mm)a,b 1.69 ± 0.24 1.06 ± 0.23 1.69 ± 0.24 1.06 ± 0.23 1.69 ± 0.24
Density at 1 div (×103 cells/mm2)c 2.5 ± 1.5 1.6 ± 0.6 0.60 ± 0.24 0.30 ± 0.16 0.11 ± 0.06
Number of cultures followed 30 12 10 3 3
Number of batches 8 3 3 1 1
  1. aMean ± sample standard deviation, N = 3 measured drops each of 5 and 20 μL.
  2. bMeasured by focusing an inverted microscope on surface of MEA and top of drop (made visible by sprinkling some dust on it), and correcting for the refractive index of the liquid.
  3. cMean ± sample standard deviation, based on N = 8, 8, 7, 3, 3 cultures. Cells in the central 0.36 mm2 of each MEA were counted using digital images.