Effects of inhibitors on FGF2-stimulated ERK and GSK3β activity Immunocomplex kinase assays for (A) ERK and (B) GSK3β activity without FGF2 treatment, or after inhibition with PD98059, U0126, LY29004, Bisindolymaleimide I or Gö6983 followed by FGF2 treatment. (C) Inhibitor treatment alone. (D) Summary of changes in phosphorylation (P) versus changes in activity (Act) of ERK and GSK3β. ⇑ Indicates an increase, ⇓ indicates a decrease, – indicates no change. * = P < 0.05 by One-Way ANOVA with post-hoc Dunnett's when compared to control.