Effects of inhibitors on FGF2-mediated ERK activation A) Western blot (WB) reacted with anti-phospho-ERK after treatment with FGF2 for 0, 5, 10 min (lanes 1–3) and after inhibitors LY294002 (to block PI3K), U0126 (to block MEK), and Bis I and Gö6983 (to block PKC) (lanes 4–7, respectively). B) WB reacted with anti-total ERK antibody. (C) WB reacted with anti-phospho-GSK3β. D) WB reacted with anti-GSK3β. E) WB reacted with anti-PI3K antibody. The same WB was used in each experiment after stripping, reblocking and incubating with new antibodies for the given protein.