Table 1 Primers and oligonucleotide probes used for confirmation of results.
From: Genomic responses in rat cerebral cortex after traumatic brain injury
Gene | Primer/oligonucleotide probe sequence (nts) | Product (bp) | Acc. no |
|---|---|---|---|
PAT | F: 5'-GAAGCTAGAGGCTGAGGATC- 3' R: 5'-GTGCAGTCTCCCTCCTTCAC- 3' | 233 | L00091 |
S-100 | F: 5'-GGACCTGAGAGTGCTCATGG- 3' R: 5'-GCATGCAATGATGAGCCCCG-3' | 222 | J03627.1 |
IGF-II | F: 5'-GACTGAGTTGGGGCAAATAC- 3' R: 5'-CAGGTGTTAGGAAGGTGCTC- 3' | 211 | AA899788 |
CD-44 | F: 5'-CCGACCTTCCCACTTCACAG- 3' R: 5'-TCTCCTCGCAGGACCAGAAG- 3' 5'- ATCACTGGTGGCCAGGGTGCTCCCA ATAAAGAAGGCGTCATCCC-3' (44-mer) | 200 | AA817820 |
OPN | F: 5'-CTGCCAGCACACAAGCAGAC- 3' R: 5'-ACTCCTTGGACTGCTCCAGG- 3' 5'-TCCTGATCAGAGGGCACGCTCAGAC GCTGGGCAACTGGGATGACC-3' (45-mer) | 323 | M14656 |
TIMP-1 | F: 5'-CGAGACCACCTTATACCAGCG-3' R: 5'-CAGGAAGCTGCAGGCAGTGAT-3' | 216 | L31883 |
TIMP-2 | F: 5'-TGCACCCGCAACAGGCGTTTT-3' R: 5'--TTCCTCCAACGTCCAGCGAGA-3' | 224 | L31884 |
VTN | F: 5'-ATCGACGCTGCCTTCACTCG-3' R: 5'-TGGCGCCATCAGAGGATCTG-3' | 373 | U44845 |