Fluorescence photomicrographs showing CD-44 and osteopontin immunoreactivity, 4 dpi. High-power photomicrographs of injured rat brains close to impact show immunofluorescence staining for CD-44 and osteopontin (OPN). Nuclear staining with Hoescht illustrates, in the merged picture, (CD-44/Hoescht) the cytoplasmatic staining for CD-44. Immunofluorescence staining for ED-1, a macrophage marker, demonstrates, in the merged picture, (OPN/ED-1) co-localisation with osteopontin. Scale bar = 50 μm.