Figure 3

Constitutive SDF-1 protein expression at P8. The left column shows SDF-1 antibody staining of P8 mouse brain (Cy3 – red). The middle column shows the corresponding nuclear labelling (bis-benzimide – blue) of the same sections. The right hand column shows the merged images. Panels A-C show the distribution of constitutive SDF-1 expression at low power. The arrows denote diffuse labelling over the neocortex. Panels C-E demonstrate the hippocampal SDF-1 expression pattern. Panels G-I show a high magnification of the hippocampal fissure down through the upper blade of the dentate gyrus granular cell layer. The arrowheads point out SDF-1 positive blood vessels. Abbreviations: ca – cornus ammons (CA1, 2, 3), cp – choroid plexus, dhc – dorsal hippocampal commissure, ec – external capsule, fi – fimbria hippocampus, fmj – forceps major corpus callosum, GrDG – granular layer dentate gyrus, hif – hippocampal fissure, LMol – lacunosum moleculare layer hippocampus, Mol – molecular layer dentate gyrus, Or – oriens layer, PoDG – polymorph layer dentate gyrus, Rad – stratum radiatum. All Scale bars equal 200 μm.