SDF-1 and RCA-1 Co-localization. Figure shows RCA and SDF-1 dual labeling in hippocampus from P10 mice three days post-HI; RCA-1 labeling in hippocampus (A & D), SDF-1 expression in hippocampus (B & E), merged RCA-1 and SDF-1 labeling (C & F). Panels A-C represent 20× objective fields. Panels D-E are single z plane fields and panel F is a merged orthogonal view of a confocal z stack. The arrowhead points out an example of an SDF-1 negative RCA-1 positive cell. Scale bar equals 50 μm.