P75NTR regulation following olfactory bulb lesions: P75NTR expression was determined by Western blot assays (A) and receptor expression was quantified and normalized to a loading control (JNK) in the histogram (B). P75NTR was increased in lesioned animals, and estrogen treatment for 3 weeks prior to the lesion further enhanced the expression of this receptor. Two representative examples are shown of each treatment condition in the Western blots. Bars represent mean ± SEM, n = 6 per group. (C) Specificity of p75NTR antibody: The antibody used in this Western assay recognizes a size appropriate band at 75 kD, which is not seen when the membrane is probed in the absence of the primary antibody. Lane 1: PC12 cell lysate, Lanes 2,3: olfactory bulb lysate. Key: OVX Sham: Ovariectomized female replaced with a control pellet, with sham injection, OVX lesion: ovariectomized female replaced with a control pellet, with NMDA injection, Est Sham: ovariectomized female replaced with an estrogen pellet, with sham injection, Est Lesion: ovariectomized female replaced with an estrogen pellet, with NMDA injection. *: p < 0.05.